The Expression of Wnt5a Gene Throughout Definitive Endoderm Induction Process in Induced Pluripotent Stem Cells
نویسندگان
چکیده
منابع مشابه
differentiation of definitive endoderm from human induced pluripotent stem cells on hmscs feeder in a defined medium
background: the definitive endoderm (de) differentiation using the undefined media and non-human feeders can cause contaminations in the generated cells for therapeutic applications. therefore, generating safer and more appropriate de cells is needed. this study compared five different methods to establish an appropriate method for inducing an efficient de differentiation from human induced plu...
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Pluripotent stem cells present an extraordinary powerful tool to investigate embryonic development in humans. Essentially, they provide a unique platform for dissecting the distinct mechanisms underlying pluripotency and subsequent lineage commitment. Modest information currently exists about the expression and the role of ion channels during human embryogenesis, organ development, and cell fat...
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Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell sources for regeneration of damaged tissues and organs or treatment for incurable diseases. Human embryonic stem cells (HESCs) hold two important properties called self renewal and pluripotency. However, the use of ...
متن کاملSecreted Cerberus1 as a Marker for Quantification of Definitive Endoderm Differentiation of the Pluripotent Stem Cells
To date, CXCR4 and E-cadherin double-positive cells detected by flow cytometry have been used to identify the differentiation of embryonic stem (ES) cells or induced pluripotent stem (iPS) cells into definitive endoderm (DE) lineages. Quantification of DE differentiation from ES/iPS cells by using flow cytometry is a multi-step procedure including dissociation of the cells, antibody reaction, a...
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objective: in vitro production of a definitive endoderm (de) is an important issue in stem cell-related differentiation studies and it can assist with the production of more efficient endoderm derivatives for therapeutic applications. despite tremendous progress in de differentiation of human embryonic stem cells (hescs), researchers have yet to discover universal, efficient and cost-effective ...
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ژورنال
عنوان ژورنال: Gene, Cell and Tissue
سال: 2020
ISSN: 2345-6833,2345-6841
DOI: 10.5812/gct.110381